TB-500 Reconstitution Calculator: BAC Water Volumes & Concentration Tables for Lab Research
DISCLAIMER: TB-500 (Thymosin Beta-4 fragment) is a research chemical intended exclusively for qualified laboratory use in preclinical and in vitro research settings. It is not approved by the FDA for use in humans or animals. All concentration calculations described on this page are provided solely for laboratory research purposes. Nothing herein constitutes medical advice, dosing guidance, or instructions for self-administration. Always follow your institution's standard operating procedures and biosafety requirements.
TB-500 Reconstitution Calculator: BAC Water Volumes & Concentration Tables for Lab Research
Last Updated: May 2026 | Reading Time: ~8 minutes | Author: Palmetto Peptides Research Team
TB-500 is one of the most studied tissue repair peptides in preclinical research, and it is frequently used in protocols that require careful concentration control across multiple experimental timepoints. Reconstitution errors — using the wrong volume of bacteriostatic water — cascade into every downstream measurement: in vitro assay concentrations, dosing schedules in animal models, and statistical reproducibility across experiments.
This page provides concentration lookup tables for Palmetto Peptides' 5mg and 10mg TB-500 vials. The tables show exactly how much BAC water to add for each common working concentration, eliminating the need to recalculate manually each time.
For the full step-by-step reconstitution protocol — equipment, aseptic technique, injection method, and troubleshooting — see our BPC-157 and TB-500 Reconstitution Protocols guide. This page focuses on the concentration math only.
TB-500: Research Background
TB-500 is a synthetic peptide fragment of Thymosin Beta-4, a naturally occurring protein with roles in actin sequestration, cell migration, and wound response signaling. In preclinical research, TB-500 has been studied in tendon, muscle, cardiac, and corneal tissue models, with particular focus on angiogenesis and extracellular matrix remodeling. It is often studied alongside BPC-157 in combined tissue repair research protocols.
Palmetto Peptides supplies TB-500 as lyophilized powder in two standard vial sizes: 5mg and 10mg, each with third-party HPLC purity verification (≥98%) and mass spectrometry identity confirmation.
View TB-500 Research Peptide →
The Reconstitution Formula
Volume to add (mL) = Peptide mass (mg) ÷ Target concentration (mg/mL)
Always use your COA fill weight — not the labeled amount — for precise calculations.
TB-500 Concentration Calculator — 5mg Vial
| Target Concentration | BAC Water to Add | Total Solution Volume |
|---|---|---|
| 0.5 mg/mL | 10.0 mL | 10.0 mL |
| 1.0 mg/mL | 5.0 mL | 5.0 mL |
| 2.0 mg/mL | 2.5 mL | 2.5 mL |
| 5.0 mg/mL | 1.0 mL | 1.0 mL |
Based on nominal fill weight of 5.0mg. Adjust using actual COA fill weight.
TB-500 Concentration Calculator — 10mg Vial
| Target Concentration | BAC Water to Add | Total Solution Volume |
|---|---|---|
| 0.5 mg/mL | 20.0 mL | 20.0 mL |
| 1.0 mg/mL | 10.0 mL | 10.0 mL |
| 2.0 mg/mL | 5.0 mL | 5.0 mL |
| 5.0 mg/mL | 2.0 mL | 2.0 mL |
Based on nominal fill weight of 10.0mg. Adjust using actual COA fill weight.
Adjusting for Actual COA Fill Weight
If your COA reports a fill weight different from the nominal amount:
Example: COA fill weight = 9.76mg, target = 2.0 mg/mL Volume to add = 9.76 ÷ 2.0 = 4.88 mL BAC water
This adjustment ensures concentration accuracy regardless of fill variation, which is especially important in quantitative assay work.
Solvent Selection
Bacteriostatic water (0.9% benzyl alcohol) is the recommended solvent for TB-500 research solutions. The benzyl alcohol preservative extends usable shelf life of the reconstituted solution and is compatible with TB-500's peptide chemistry.
Sterile water for injection is acceptable only for immediate single-use protocols. It has no preservative properties and should not be used for solutions stored across multiple research sessions.
Palmetto Peptides supplies Bacteriostatic Water (BAC Water) 10mL vials for research reconstitution.
Aliquot Volume Reference
| Vial Size | Concentration | Total Volume | 100μL Aliquots |
|---|---|---|---|
| 5mg | 1.0 mg/mL | 5.0 mL | 50 aliquots |
| 5mg | 2.0 mg/mL | 2.5 mL | 25 aliquots |
| 10mg | 1.0 mg/mL | 10.0 mL | 100 aliquots |
| 10mg | 2.0 mg/mL | 5.0 mL | 50 aliquots |
Storage Conditions
| Form | Recommended Storage | Notes |
|---|---|---|
| Lyophilized (unreconstituted) | -20°C, desiccated | Stable for 24+ months when properly stored |
| Reconstituted in BAC water | 2–8°C, protected from light | Follow lab SOPs for peptide solution stability |
| Aliquoted samples | -20°C | Thaw only what is needed; minimize freeze-thaw cycles |
Frequently Asked Questions
How much BAC water for a 5mg TB-500 vial at 1 mg/mL? Add 5.0 mL of bacteriostatic water. This yields 5.0 mL of 1 mg/mL TB-500 research solution.
How much BAC water for a 10mg TB-500 vial at 2 mg/mL? Add 5.0 mL. Using the formula: 10 ÷ 2 = 5.0 mL.
Does TB-500 dissolve readily in BAC water? Lyophilized TB-500 is generally soluble in aqueous solvents. If the cake does not dissolve immediately, gentle rolling or inversion for 60–90 seconds is typically sufficient. Avoid vortexing, which can shear peptide structure.
Can I store reconstituted TB-500 in a standard lab refrigerator? Yes. Store at 2–8°C protected from light. The benzyl alcohol in bacteriostatic water provides preservative action during refrigerated storage. Follow your laboratory's SOPs for documented stability windows.
What is the difference between TB-500 and Thymosin Beta-4? TB-500 is a specific synthetic fragment of the full Thymosin Beta-4 protein (typically the Ac-LKKTETQ or related actin-binding region), not the full-length protein. Most preclinical research literature uses "TB-500" and "Thymosin Beta-4 fragment" interchangeably for this synthetic version.
Source Your TB-500 from Palmetto Peptides
Concentration accuracy begins with mass accuracy. Palmetto Peptides provides lot-specific COA data with actual fill weights so your reconstitution calculations are grounded in verified numbers rather than labeled estimates.
Order TB-500 Research Peptide →
Related research tools:
- BPC-157 Research Peptide — commonly studied alongside TB-500
- BPC-157 + TB-500 Wolverine Stack — pre-matched vial set for combined tissue repair research
- Bacteriostatic Water (BAC Water) — pharmaceutical-grade reconstitution solvent