CJC-1295 Reconstitution Calculator: BAC Water Volumes & Concentration Tables for Lab Research
DISCLAIMER: CJC-1295 is a research chemical intended exclusively for qualified laboratory use in preclinical and in vitro research settings. It is not approved by the FDA for use in humans or animals. All concentration calculations described on this page are provided solely for laboratory research purposes. Nothing herein constitutes medical advice, dosing guidance, or instructions for self-administration. Always follow your institution's standard operating procedures and biosafety requirements. Palmetto Peptides supplies research-grade peptides for scientific investigation in compliance with all applicable law.
CJC-1295 Reconstitution Calculator: BAC Water Volumes & Concentration Tables for Lab Research
Last Updated: May 2026 | Reading Time: ~9 minutes | Author: Palmetto Peptides Research Team
CJC-1295 is one of the most studied GHRH (growth hormone-releasing hormone) analog peptides in preclinical research and is available in two distinct variants — CJC-1295 with DAC and CJC-1295 without DAC — each with different half-life profiles and research applications. Both variants are supplied as lyophilized powder and reconstituted using the same mathematical approach, but researchers new to the compound often have questions about whether the variants differ in reconstitution procedure.
This page provides concentration lookup tables for both CJC-1295 No DAC (5mg and 10mg) and CJC-1295 DAC (5mg) vials, with pre-calculated BAC water volumes for every common working concentration. It also clarifies the reconstitution differences — and lack thereof — between the two variants.
For the full step-by-step reconstitution protocol covering equipment, aseptic technique, and troubleshooting, see our Step-by-Step CJC-1295 Reconstitution Protocol guide. This page covers the concentration math only.
CJC-1295: Research Background
CJC-1295 is a synthetic analog of growth hormone-releasing hormone (GHRH) designed to stimulate pituitary growth hormone secretion in preclinical research models. It exists in two forms with distinct pharmacokinetic profiles:
- CJC-1295 No DAC — shorter acting variant, producing pulsatile GH release patterns in animal models; widely used in research requiring episodic hormone stimulation
- CJC-1295 with DAC (Drug Affinity Complex) — modified with a lysine-DAC attachment that binds albumin, dramatically extending half-life; used in research designs requiring sustained GHRH signaling
The two variants are frequently studied in comparison to characterize pulsatile versus sustained GH secretion patterns. They are also often paired with Ipamorelin in combined growth hormone secretagogue research protocols.
Palmetto Peptides supplies:
- CJC-1295 No DAC — available in 5mg and 10mg vials
- CJC-1295 with DAC — available in 5mg vials
The Reconstitution Formula
Volume to add (mL) = Peptide mass (mg) ÷ Target concentration (mg/mL)
This formula applies identically to both CJC-1295 No DAC and CJC-1295 DAC. The DAC modification does not change how the peptide dissolves in aqueous solvent.
Always use your COA fill weight — not the labeled amount — for precise concentration control.
CJC-1295 No DAC Concentration Calculator — 5mg Vial
| Target Concentration | BAC Water to Add | Total Solution Volume |
|---|---|---|
| 0.5 mg/mL | 10.0 mL | 10.0 mL |
| 1.0 mg/mL | 5.0 mL | 5.0 mL |
| 2.0 mg/mL | 2.5 mL | 2.5 mL |
| 5.0 mg/mL | 1.0 mL | 1.0 mL |
Based on nominal fill weight of 5.0mg. Adjust using actual COA fill weight.
CJC-1295 No DAC Concentration Calculator — 10mg Vial
| Target Concentration | BAC Water to Add | Total Solution Volume |
|---|---|---|
| 0.5 mg/mL | 20.0 mL | 20.0 mL |
| 1.0 mg/mL | 10.0 mL | 10.0 mL |
| 2.0 mg/mL | 5.0 mL | 5.0 mL |
| 5.0 mg/mL | 2.0 mL | 2.0 mL |
Based on nominal fill weight of 10.0mg. Adjust using actual COA fill weight.
CJC-1295 DAC Concentration Calculator — 5mg Vial
| Target Concentration | BAC Water to Add | Total Solution Volume |
|---|---|---|
| 0.5 mg/mL | 10.0 mL | 10.0 mL |
| 1.0 mg/mL | 5.0 mL | 5.0 mL |
| 2.0 mg/mL | 2.5 mL | 2.5 mL |
| 5.0 mg/mL | 1.0 mL | 1.0 mL |
Based on nominal fill weight of 5.0mg. Adjust using actual COA fill weight. Tables are identical to No DAC 5mg — the peptide mass and solvent are the same; only the biological half-life differs.
DAC vs. No DAC: What Changes and What Doesn't
| Factor | CJC-1295 No DAC | CJC-1295 DAC |
|---|---|---|
| Reconstitution formula | Volume = mass ÷ concentration | Identical |
| Preferred solvent | Bacteriostatic water | Identical |
| Storage (lyophilized) | -20°C | Identical |
| Storage (reconstituted) | 2–8°C | Identical |
| Available vial sizes | 5mg, 10mg | 5mg only |
| Biological half-life in models | Short (pulsatile GH pattern) | Extended (albumin-bound) |
| Research application | Episodic GH secretion studies | Sustained GHRH signaling studies |
The DAC modification is a research design consideration — not a reconstitution one.
Adjusting for Actual COA Fill Weight
Example: COA fill weight = 4.93mg, target = 1.0 mg/mL Volume to add = 4.93 ÷ 1.0 = 4.93 mL BAC water
In combination protocols (CJC-1295 + Ipamorelin) where two peptides are reconstituted separately and combined, fill-weight accuracy in each vial ensures the concentration ratio in the combined preparation is correctly controlled.
Solvent Selection
Bacteriostatic water (0.9% benzyl alcohol) is the standard reconstitution solvent for CJC-1295 research solutions. Preferred for any protocol where the reconstituted solution will be stored between experimental uses.
Sterile water for injection is acceptable only for immediate single-use applications without preservative requirements.
Palmetto Peptides supplies Bacteriostatic Water (BAC Water) 10mL vials for research reconstitution.
Aliquot Volume Reference
| Vial | Variant | Concentration | Total Volume | 100μL Aliquots |
|---|---|---|---|---|
| 5mg | No DAC or DAC | 1.0 mg/mL | 5.0 mL | 50 aliquots |
| 5mg | No DAC or DAC | 2.0 mg/mL | 2.5 mL | 25 aliquots |
| 10mg | No DAC | 1.0 mg/mL | 10.0 mL | 100 aliquots |
| 10mg | No DAC | 2.0 mg/mL | 5.0 mL | 50 aliquots |
Storage Conditions
| Form | Recommended Storage | Notes |
|---|---|---|
| Lyophilized (unreconstituted) | -20°C, desiccated | Stable for 24+ months when properly stored |
| Reconstituted in BAC water | 2–8°C, protected from light | Follow lab SOPs for peptide solution stability |
| Aliquoted samples | -20°C | Limit freeze-thaw cycles |
Frequently Asked Questions
How much BAC water for a 5mg CJC-1295 vial at 1 mg/mL? Add 5.0 mL. This applies to both No DAC and DAC variants.
How much BAC water for a 10mg CJC-1295 No DAC vial at 2 mg/mL? Add 5.0 mL. Formula: 10 ÷ 2 = 5.0 mL.
Do I need different equipment to reconstitute CJC-1295 DAC vs. No DAC? No. The reconstitution procedure, equipment, and solvent are identical. The DAC modification affects biological behavior in research models, not the chemistry of dissolving the lyophilized peptide.
Can CJC-1295 and Ipamorelin be reconstituted together in one vial? Many researchers reconstitute them separately and combine in a single syringe at point of use. This maintains independent concentration control for each peptide. Co-reconstitution in a single vial is possible but complicates concentration verification.
Is CJC-1295 No DAC or DAC better for research? This depends entirely on your research question. No DAC produces pulsatile, short-duration GH stimulation patterns; DAC produces sustained, albumin-bound signaling. See our CJC-1295 DAC vs. No DAC research comparison guide for a detailed breakdown.
Source Your CJC-1295 from Palmetto Peptides
Palmetto Peptides supplies both CJC-1295 variants with third-party HPLC purity verification (≥98%), mass spectrometry identity confirmation, and lot-specific COA documentation.
Related research tools:
- Ipamorelin Research Peptide — selective GHRP frequently paired with CJC-1295 in GH secretagogue research
- Sermorelin Research Peptide — endogenous GHRH analog for comparative GH axis research
- Bacteriostatic Water (BAC Water) — pharmaceutical-grade reconstitution solvent