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The Chemical Structure of SS-31 Research Peptide: Sequence, Molecular Formula, and Molecular Weight Explained

Aubrey Walker
April 21, 2026
ss-31research peptidespeptide chemistry

Research Notice: This article covers research on SS-31 research peptide and MOTS-C research peptide — available from Palmetto Peptides for laboratory use only.

Research Disclaimer: SS-31 is sold by Palmetto Peptides strictly as a research compound for in vitro and laboratory use only. It is not intended for human or veterinary consumption, administration, or therapeutic use.

SS-31 is a tetrapeptide research compound with the sequence D-Arg-Dmt-Lys-Phe-NH2, molecular formula C32H49N9O5, and molecular weight approximately 639.80 g/mol. Researchers can order SS-31 research peptide from Palmetto Peptides with full lot-specific COA documentation for mitochondrial laboratory studies.

The SS-31 Amino Acid Sequence, Position by Position

Position 1: D-Arginine (D-Arg)

The D-form of arginine contributes a positive charge at physiological pH via its guanidinium side chain. The D-configuration provides resistance to proteolytic degradation in cell-based assay work — a practical advantage over standard L-peptides.

Position 2: 2',6'-Dimethyltyrosine (Dmt)

A synthetic non-proteinogenic amino acid derived from tyrosine with two methyl groups added to the aromatic ring. This increases lipophilicity and membrane affinity, and is central to the cardiolipin-binding behavior studied in mitochondrial research. Researchers exploring related mitochondrial science may also find MOTS-C mitochondrial peptide research and mitochondrial function research relevant.

Position 3: Lysine (Lys)

Standard L-lysine contributes a positive charge at physiological pH, completing the alternating aromatic-cationic motif that defines the Szeto-Schiller family.

Position 4: Phenylalanine-NH2 (Phe-NH2)

The C-terminal amide cap eliminates the negative charge at the terminus, resulting in net charge approximately +3 at physiological pH.

Molecular Formula and Molecular Weight Reference

PropertyValue
Molecular FormulaC32H49N9O5
Molecular Weight~639.80 g/mol
Net Charge (pH 7.4)+3
CAS Number736992-21-5
Also Known AsElamipretide, MTP-131, Bendavia

Why This Formula Matters in Laboratory Research

The formula C32H49N9O5 is the foundation for mass spectrometry identity verification. The nitrogen-rich composition (9 nitrogen atoms) reflects the multiple guanidinium and amino groups driving the positive charge. For additional context: NAD+ research peptide science and cellular health research. Order SS-31 research peptide here.

Frequently Asked Questions

What is the amino acid sequence of SS-31?

D-Arg-Dmt-Lys-Phe-NH2, where Dmt is 2',6'-dimethyltyrosine.

What is the molecular formula of SS-31?

C32H49N9O5, molecular weight approximately 639.80 g/mol.

Is SS-31 approved for human use?

No. SS-31 has not received FDA approval. It is available exclusively as a research compound for qualified laboratory settings.

See Also: SS-31 Research Peptide: Complete Guide

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